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. 2017 Mar 10;5:12. doi: 10.3389/fchem.2017.00012

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Copyright © 2017 Czapiński, Kiełbus, Kałafut, Kos, Stepulak and Rivero-Müller.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SynNotch system. Activation of Notch by its ligand (DLL) leads to mechanical forces and further proteolytical (scissors) cleavage of Notch extracellular domain (NICD) which then translocates to the nucleus and modulates gene expression. SynNotch exploits the mechanism of Notch receptor activation, using an antibody as an extracellular domain fused to the transmembrane domain (TMD) of Notch. Instead of NICD, different transcription factors can be used. The mechanical forces between the receptor (antibody) and the cell- or surface-attached ligand (antigen) resulted in the release of the intracellular domain (transcription factor) and the transcription of specific genes (reporters).