FIGURE 2.

Developmental Changes in the Expression of GABA Neuron Markers and Chloride Cotransporters Transcripts in Dysbindin Null Hippocampus. (A) mRNA was isolated from wild type and Bloc1s8^sdy/sdy embryonic day 18, newborn (P0), and postnatal day 14 (P14) hippocampi. Transcripts were quantified by real time quantitative PCR for the indicated GABA neuron gene products and the chloride transporters NKCC1 and KCC2. (B) Shows a similar analysis performed in prefrontal cortex. Tissue collections and processing were performed for each age independently, thus qRT-PC comparisons were performed within a specific age. Wild type values for a transcript were defined as 1 at each specified age. All determinations were performed from at least three animals per genotype and two independent cDNA preparations for qRT-PCR. Comparisons between wild type and Bloc1s8^sdy/sdy were performed with Wilcoxon–Mann–Whitney Rank Sum Test. All comparisons were significantly different with a p < 0.00041 unless specified otherwise as not significant (NS).