Fig. 1.

BA induces a lower polysome/monosome ratio in DU-145 cells indicating a reduction in global protein translation. DU-145 cells treated with 10 μM BA for 2 h (a) had a significantly lower polysome/monosome ratio than did DU-145 cells treated with 0 μM BA for 2 h (b). Polysomes from untreated and BA-treated cells were obtained from cells treated in parallel using paired culture plates and centrifuging together in the same rotor. Figure shows a representative replicate. The difference in polysome/monosome ratios from three independent experiments was evaluated using a paired t test (p < 0.001, n = 3)