Fig. 5.

BA induces an increase in ATF4 transcription in DU-145 cells. Ten micromoles of BA induced a significant increase in ATF4 mRNA 1 (p = 0.031, n = 4) and 2 h (p = 0.005, n = 4) post-treatment. One micromole of thapsigargin (Tg) and DMSO vehicle (DM) was used as a positive control and significantly induced ATF4 mRNA, p < 0.05 (n = 5). The gel shown is a representative replicate. A description of the statistical analysis is given in the legend of Fig. 2