Fig. 6.

BA induces an increase in ATF4 protein in DU-145 cells. a DU-145 cells were treated with 10 μM BA for 0, 0.25, 0.5, 1, 2, 3, 4, 5, and 6 h. ATF4 protein was significantly increased in cells treated at 1 (p = 0.01, n = 4), 2 (p < 0.05, n = 3), and 3 h (p = 0.004, n = 3) and decreased at 5 h (p < 0.030, n = 4) post-treatment. b ATF3 protein was significantly increased in DU-145 cells treated with 1 μM thapsigargin (Tg) or DMSO (DM) for 1 h, (p < 0.05 n = 3). The gel shown is a representative replicate. A description of the statistical analysis is given in the legend of Fig. 2