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. 2017 Mar 8;8:14713. doi: 10.1038/ncomms14713

Figure 2. PpCYP98 is essential for P. patens gametophore development.

Figure 2

(a–d) GUS staining pattern in PpCYP98:uidA lines indicated a prominent expression in developing gametophores. (a) Bud (emerging gametophore). Scale bar, 50 μm. (b) Young gametophores. Scale bar, 0.5 mm. (c) One-month-old gametophore. Scale bar, 2 mm. (d) Apical leaf from 1-month-old gametophore (joined pictures). No GUS staining is visible in the midrib. Scale bar, 0.5 mm. (e–j) ΔPpCYP98 mutants fail to develop normal gametophores. (e,f) Six-week-old colonies grown on agar plates. Arrowheads indicate gametophores. Scale bars, 5 mm. (g–h) Close-up views of gametophores. Scale bars, 0.5 mm. (i,j) Toluidine blue-stained cross section of gametophores. Arrowheads indicate phyllids in the WT. Scale bars, 0.1 mm.