Table 1. Characterization of expanded mononuclear cells from rat umbilical cord blood.
0 DIV | 10 DIV | proliferation (times) | |
---|---|---|---|
Total cell number(per litter) (x 106 cells) | 0.72 ± 0.18a(n = 6) | 10.7 ± 1.5a(n = 5) | 16.4 ± 2.4 |
CFU-GM assay (%)(% of colony-forming cells) | 0.90 ± 0.12b(n = 5) | 20.2 ± 1.04b(n = 4) | 22.7 ± 4.9 |
Total colony-forming cells(per litter) (x 104 cells) | 0.77 ± 0.21(n = 5) | 234 ± 40.4(n = 4) | 433 ± 87.3 |
CD133+/DAPI (%) | 54.7 ± 10.8c(n = 4) | 77.0 ± 8.1c(n = 4) | |
CD34+/DAPI (%) | 16.4 ± 4.5d(n = 4) | 3.8 ± 0.3d(n = 4) |
Data are expressed as means ± SEM. a,bp < 0.001, cNot significant, dp < 0.05.