Skip to main content
. 2017 Mar 10;7:44328. doi: 10.1038/srep44328

Figure 6. Plectin regulates Cav-1 enrichment and PKA activity in RasV12-transformed cells surrounded by normal epithelial cells.

Figure 6

(a) Effect of plectin-knockdown on Cav-1 accumulation. (c) Effect of Cav-1-knockdown on plectin accumulation. (e) Effect of plectin-knockdown on activity of PKA. (a,c,e) MDCK-pTR GFP-RasV12 cells, MDCK-pTR GFP-RasV12 plectin-shRNA1 cells, or MDCK-pTR GFP-RasV12 Cav-1-shRNA1 cells were mixed with normal MDCK cells on collagen gels. Cells were fixed after 16 h incubation with tetracycline and stained with anti-plectin antibody (white) with anti-Cav-1 (red) or anti-PKA-substrate (red) antibody, and Hoechst (blue). Scale bars, 10 μm. (b,d,f) Quantification of (a,c,e). Data are mean ± SD from three independent experiments. n = 30 cells for each experimental condition. (b) *P < 0.0005, (f) *P < 0.005. Values are expressed as a ratio relative to plectin-shRNA1 (−) (b,f) or Cav-1-shRNA1 (−) (d). (g) The mode of action of the plectin-EPLIN-microtubules complex in apical extrusion of RasV12-transformed cells.