Figure 1. Experimental design to compare variability between flow cytometers.

Flow chart of the experimental set up outlining the establishment and verification of the NK cell phenotype and function panels to compare variability between flow cytometers. Following selection of backbone and drop-in antibodies, reference samples were used for optimizing and checking fluorochrome intensities using appropriate single stains and fluorescence minus one (FMO) controls. Further, the data generated from three instruments were analysed using KALUZA^® software and results were tabulated using Graphpad Prism for statistical differences for validation using the same reference sample.