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. 2017 Mar 10;7:43873. doi: 10.1038/srep43873

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The experimental set up describes the work flow to compare NK phenotype and function changes under different PBMC (non-stimulated and stimulated) conditions. PBMC from 12 healthy donors were stained with NK cell phenotype and NK function panel cocktails. Data was acquired for 6 donors using BD LSRFortessa^TM and for other 6 donors in MACSQuant^® Analyzer 10. Further, FCS files from LSRFortessa^TM were analysed on KALUZA^® and for MACSQuant^® Analyzer 10 in MACSQuantify^TM 2.8. Data obtained from both devices were combined and examined for statistical significance between fresh and cryopreserved NK cells.