Table 1. Comparison of instrument settings between three flow cytometers from three centers participated in the study.
| Comparison of instrument settings between BD FACS Canto™ II, BD LSRFortessa™ and MACS Quant® Analyzer 10 | ||||||
|---|---|---|---|---|---|---|
| Fluorochromes | Filter settings - Band pass & Long pass filters | PMT voltages | ||||
| BD FACS Canto™ II | BD LSR Fortessa™ | MACS Quant® Analyzer 10 | BD FACS Canto™ II | BD LSR Fortessa™ | MACS Quant® Analyzer 10 | |
| FSC | 488/10 | 488/10 | 488/10 | 319 | 489 | 328 |
| SSC | 488/10 | 488/10 | 488/10 | 462 | 266 | 476 |
| FL1 | 530/30 & 502LP | 530/30 & 505LP | 525/50 | 496 | 484 | 417 |
| FL2 | 585/42 & 556LP | 575/26 & 550LP | 585/40 | 435 | 473 | 416 |
| FL3 | 780/60 & 735LP | 780/60 & 750LP | 750LP | 540 | 549 | 487 |
| FL4 | 670LP & 655LP | 695/40 & 685LP | 655–730 | 507 | 681 | 594 |
| FL5 | 660/20 | 670/14 & 655LP | 655–730 | 588 | 484 | 522 |
| FL6 | 780/60 & 735LP | 780/60 & 750LP | 750LP | 474 | 451 | 579 |
| FL7 | 450/50 | 450/50 | 450/50 | 375 | 423 | 444 |
| FL8 | 510/50 & 502LP | 525/50 & 505LP | 525/50 | 403 | 453 | 560 |
Three flow cytometers (BD FACS Canto™ II, BD LSR Fortessa™ and MACS Quant® Analyzer 10) with compatible optical configuration were used for NK phenotype and NK function FACS panel design and optimization studies. Forward scatter (FSC), side scatter (SSC) and eight fluorescence emission channels (FL1-FL8) were used. Further, their corresponding band pass and long pass (LP) filter settings were compared and the photomultiplier tube (PMT) voltages obtained are mentioned.