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. 2017 Mar 10;7:43873. doi: 10.1038/srep43873

Table 2. Antibody specifications - NK phenotype panel.

Laser Antibody Fluorochrome Clone Titration Manufacturer Catalogue No
Violet 405 nm CD45 VioGreen 5B1 1:11 Miltenyi 130-096-906
CD3 VioBlue BW264/56 1:11 Miltenyi 130-094-363
TCRγδ VioBlue 11F2 1:11 Miltenyi 130-101-557
CD14 VioBlue TÜK4 1:11 Miltenyi 130-094-364
CD19 VioBlue LT19 1:11 Miltenyi 130-098-598
Sytox® Blue Dead cell marker   1:1000 Life technologies S11348
Blue 488 nm PanKIR2D FITC NKVFS1 1:11 Miltenyi 130-098-689
NKG2A PE-Vio770 REA110 1:11 Miltenyi 130-105-647
NKG2C PE REA205 1:11 Miltenyi 130-103-635
NKG2D PerCP-Cy5.5 1D11 1:11 Biolegend 320818
Red 633 nm CD16 APC VEP13 1:11 Miltenyi 130-091-246
CD56 APC-Vio770 REA196 1:11 Miltenyi 130-100-694

Antibody-fluorochrome conjugates for NK phenotype panel were distributed across Violet, Blue and Red lasers. Backbone antibodies (Bold) were assigned to Violet and Red lasers. VioGreen was used to gate on CD45+ cells and VioBlue in the Violet laser was used as a dump channel to exclude non-NK lymphocytes including dead cells. Red laser was used to gate on viable NK cells, followed by NK phenotype panel drop-in markers (white shades) in the Blue laser. All antibodies except Sytox Blue (1:1000) were used at a dilution of 1:11.