Table 5. Comparison of NK phenotype and function parameters between non-activated fresh and cryopreserved samples.
Non-activated fresh versus cryopreserved NK samples (Wilcoxon test) | |||
---|---|---|---|
NK FACS panel drop-ins | NK only | NK+A431 | NK+A431+CET |
CD56+NKG2A PE-Vio770 | ns | ns | ns |
CD56+NKG2C PE | ns | ns | ns |
CD56+NKG2D PerCP-Cy5.5 | ns | ns | ns |
CD56+PanKIR2D FITC | ns | ns | ns |
CD56+CD107a PE | ns | ns | ns |
CD56+CD16 APC | ns | ns | ns |
CD56+NKp44 PE Vio770 | ns | ns | ns |
CD56+CD25 VioBrightFITC | ns | ns | ns |
Non-activated freshly isolated NK cell samples from 12 healthy donors and their corresponding cryopreserved counterparts were stained with the NK phenotype and NK function antibody mix and compared under different stimulation conditions (NK only, NK+A431, NK+A431+CET). All samples were measured in triplicates. Statistical analysis to detect differences between non-activated fresh and cryopreserved samples was performed using the Wilcoxon test. No significant p-values (<0.05) were obtained for the assessed NK phenotype and function panel drop-in parameters. Marker expression level of individual donors are shown in supplementary Figs S4, S5 and S8B.