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. 2017 Mar 10;7:44245. doi: 10.1038/srep44245

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Copyright © 2017, The Author(s)

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(A) The expression of GSK-3β was silenced in RAW264.7 cells using a lentiviral-based shRNA. shLuc was used as the negative control. Western blotting analysis determined the expression of GSK-3β. (B) shLuc- or shGSK-3β-transfected RAW264.7 cells were treated with ZA (100 μM) for 2 days. The percentage of apoptotic cells was detected using PI-based flow cytometry. Western blotting analysis determined the expression of Mcl-1 (C), cleaved caspase-3 (C. Caspase-3), and caspase-3 (D) in shLuc- or shGSK-3β-transfected RAW264.7 cells after ZA treatment for 3 days. β-actin was used as an internal control. One representative data set with an optical density from three individual experiments is shown. Full-length blots/gels are presented in Supplementary Figure 5.