. 2017 Mar 10;7:44201. doi: 10.1038/srep44201

Table 2. Intrinsic activities and potencies of the five most active VS hits determined at the dopamine receptor subtype D_2S by measuring the inhibition of forskolin-stimulated cAMP accumulation and recruitment of β-arrestin-2 after stimulation of the D_2S receptor expressed in HEK cells^a.

Compound	Inhibition of forskolin-stimulated cAMP accumulation		β-Arrestin recruitment^b
Compound	EC₅₀ (nM)	E_max (%)^c	EC₅₀ (nM)	E_max (%)^d
Delphinidin	N/A	20% at 100 μM	N/A	<7
Fumigaclavine A	250	27	N/A	<7
Hordenine	3700	76	N/A	<7
Salsolinol	630	21	N/A	<7
Robenidine	N/A	<5	N/A	<7
Dopamine	0.97	100	420	96

^aEC₅₀ and E_max values were derived from mean curves based on 3–7 individual dose-response curves.

^bD_2S-mediated recruitment of β-arrestin-2 determined with the PathHunter assay.

^cMaximum effect of forskolin-stimulated cAMP inhibition relative to the effect of quinpirole.

^dMaximum effect of D_2S-mediated β-arrestin recruitment relative to the maximum effect of quinpirole. N/A: EC₅₀ values could not be analysed because of low corresponding E_max values.

Table 2. Intrinsic activities and potencies of the five most active VS hits determined at the dopamine receptor subtype D2S by measuring the inhibition of forskolin-stimulated cAMP accumulation and recruitment of β-arrestin-2 after stimulation of the D2S receptor expressed in HEK cellsa.

Table 2. Intrinsic activities and potencies of the five most active VS hits determined at the dopamine receptor subtype D_2S by measuring the inhibition of forskolin-stimulated cAMP accumulation and recruitment of β-arrestin-2 after stimulation of the D_2S receptor expressed in HEK cells^a.