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. 2017 Mar 10;7:41779. doi: 10.1038/srep41779

Figure 3. Effect of IL-17 on VEGF production correlated with JAK/STAT signaling pathway in human astrocytoma cell lines.

Figure 3

The expressions of JAK/STAT signaling pathway protein p-STAT1, STAT1, p-STAT3, STAT3, p-JAK2, JAK2 were determined by western blot. Cells were treated with 100 ng/ml concentrations of IL-17 for different hours (a). Efficiency of ShRNA for STAT1 and STAT3 was tested by immunoblotting using lysates from cells treated with NT shRNA or STAT1 shRNA or STAT3 shRNA and 100 ng/ml concentrations of IL-17 for 48 h (b) or incubated with 100 ng/ml concentrations of IL-17 and NT shRNA, STAT1 shRNA, STAT3 shRNA, JAK2 inhibitor AG490 for 48 h (c) Quantitative reverse transcription-PCR analysis of VEGF genes. Cells were treated with 100 ng/ml concentration of IL-17 or non-stimulated conditions for 48 h. Meanwhile cells were incubated with NT shRNA or STAT1 shRNA or STAT3 shRNA (d). Cells were treated with 100 ng/ml concentration of IL-17 or non-stimulated conditions and JAK2 inhibitor AG490 for 48 h (e). Results are expressed as the mean ± S.D. from three independent experiments. *P < 0.05 versus NT shRNA, **P < 0.01 versus NT shRNA.