Skip to main content
. 2017 Mar 10;7:44125. doi: 10.1038/srep44125

Figure 2. Effect of CAT-SKL on mammosphere formation and self-renewal capacity in CSC-enriched cultures.

Figure 2

(A–C) A mammosphere assay was used to measure the impact of CAT-SKL treatment on the numbers of mammospheres formed per 1,000 cells passaged, 3 different TNBC cell lines were investigated: MDA-MB-468, HCC70 and MDA-MB-231. (D) Confirmation that heat inactivated CAT-SKL does not have the same effect as active CAT-SKL. (E–G) Mammosphere images are representative of the effect of CAT-SKL to reduce mammosphere size using MDA-MB-468, HCC70 and MDA-MB-231 TNBC cell lines (40x magnification). (H) Effect of CAT-SKL on CSC self-renewal potential using already formed mammospheres, here allowing MDA-MB-468 mammospheres to establish and grow for five days before starting a seven-day course of CAT-SKL treatment. Results showed a marked reduction in mammosphere size (40x magnification). Each experiment was run in triplicate and panels are representative of at least 2 independent experiments, *p < 0.05; ns, not significant.