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. 2004 Nov 15;101(47):16513–16518. doi: 10.1073/pnas.0405973101

Fig. 2.

Fig. 2.

Responses of ICUE1 to changes in cellular cAMP levels. (A) FRET response of HEK-293 cells transfected with ICUE1. The first image is a YFP-only image. Pseudocolor images depict the FRET response of the reporter to ISO stimulation at various time points. (Scale bar, 10 μm.) (B) Representative emission ratio time courses of ICUE1 and the R522E mutant stimulated with 10 μM ISO followed by 10 μM propranolol and 50 μM forskolin (FsK). (C) Representative emission ratio time courses of ICUE1 stimulated with 10 μM ISO, 50 μM forskolin, 10 μM PGE1, 300 μM 8-pCPT-2′-O-Me-cAMP, and 100 μM P-(4,5-dimethoxy-2-nitrobenzyl) cAMP followed by UV uncaging. The flash signs indicate 5-s UV flashes at two different time points.