Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Nov 28;11:5. doi: 10.5334/1750-2187-11-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © 2016 The Author(s)

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC-BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. See http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effect of MAGI proteins overexpression on CRFR1-mediated ERK1/2 signaling. (A) Representative immunoblot showing ERK1/2 phosphorylation in response to 500 nM CRF treatment for 0, 2, 5, and 15 min in non-transfected (NT) HEK293 cells, and HEK293 cells transfected with HA-CRFR1 along with either pcDNA or His-MAGI-1. Also shown are corresponding immunoblots for total ERK1/2, MAGI-1 and HA-CRFR1 protein expression. (B) Densitometric analysis of ERK1/2 phosphorylation in response to 500 nM CRF treatment for 0, 2, 5, and 15 min. The data represent the mean ± SEM of four independent experiments. (C) Representative immunoblot showing ERK1/2 phosphorylation in response to 500 nM CRF treatment for 0, 2, 5, and 15 min in non-transfected (NT) HEK293 cells, and HEK293 cells transfected with HA-CRFR1 along with either pcDNA or MAGI-2. Also shown are corresponding immunoblots for total ERK1/2, MAGI-2 and HA-CRFR1 protein expression. (D) Densitometric analysis of ERK1/2 phosphorylation in response to 500 nM CRF treatment for 0, 2, 5, and 15 min. The data represent the mean ± SEM of six independent experiments. (E) Representative immunoblot showing ERK1/2 phosphorylation in response to 500 nM CRF treatment for 0, 2, 5, and 15 min in non-transfected (NT) HEK293 cells, and HEK293 cells transfected with HA-CRFR1 along with either pcDNA or MAGI-3. Also shown are corresponding immunoblots for total ERK1/2, MAGI-3 and HA-CRFR1 protein expression. (F) Densitometric analysis of ERK1/2 phosphorylation in response to 500 nM CRF treatment for 0, 2, 5, and 15 min. The data represent the mean ± SEM of six independent experiments.