Fig. 5.

ChIP-seq analysis of Sox9. a Distribution of Sox9 binding sites in genetic elements. Many Sox9 binding sites are located in enhancers, introns, and intergenic regions. b The most enriched motif within Sox9 signal peaks. c A list of Sox9 binding sites related to Wnt signaling. d, g, j, m, p Binding profiles and peak calling records of representative Wnt component genes in Sox9 ChIP-seq analysis. Red arrows indicate peak signals. e, f Knockdown and overexpression of SOX9 in cultured cells. h, k, n, q mRNA levels of Sox9 binding site-related genes. i, l, o, r mRNA levels of Sox9 binding site-related genes after knockdown and overexpression of SOX9 in cultured cells. mRNA and protein levels are expressed relative to β-actin. Mean ± SE; n ≥ 4; *P < 0.05, **P < 0.01, ***P < 0.001 by t test. N.S. not significant