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. 2017 Mar 9;8:63. doi: 10.1186/s13287-017-0500-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Seeding of SIS scaffolds with autologous USCs. a Collagen-based scaffolds derived from porcine SIS were mechanically and chemically treated to remove cells. H&E (b) and DAPI (c) staining showed that there were two to four layers of USCs attached to both sides of the 5% PAA-treated SIS scaffold after seeding. Scale bar = 200 μm