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. 2017 Mar 9;17:62. doi: 10.1186/s12870-017-1010-x

Table 2.

List of methylated proteins found in E. grandis root tissues with elevated expression of EgPRMT1 or EgPRMT10

Protein Gene No. Score Methylated Peptide Sequences Identified by Mass Spectrometry Predicted MW (kDa)
35S::PRMT1 Endoplasmin/Heat Shock protein 90 kDa Eucgr.H00972.1 6111, 162.292 531 LGIIEDASNR 93
RuBisCO large subunit- binding protein alpha Eucgr.B02532.1 2181, 89.942 424 VGAATESELEDR 69
Alpha tubulin Eucgr.B03604.1 851, 92.432 65 AIFLDLEPTVVDEVR 49
Glyceraldehyde-3-phosphate dehydrogenase Eucgr.H04673.1 4991, 164.682 66 SISISISISIRPVR 346 RLEKAATYDEIK 36
Probable aldo-keto reductase Eucgr.H04367.1 79.322 249 NRVVYAR 38
Armadillo/beta catenin repeat protein Eucgr.B00340.1 21.522 377 IKLVKLNAVSALLGMLKSR 58
40S Ribosomal Protein Eucgr.A02371.1 1171 194 VLQFAGIDDVFTSSR 29
Transaldolase family protein Eucgr.G01978.1 2771 78 RTTLHDLYER 47
Probable Aldo-keto reductase Eucgr.H04365.1 1062 106 GTPEYAR 38
ATP synthase subunit beta Eucgr.G02224.1 1221 335 DAEGQDVLLFIDNIFR 60
Beta tubulin 5 Eucgr.D01847.1 4811 155 IREEYPDR 50
Elongation factor 1-alpha Eucgr.B02473.1 1791 244 IGGIGTVPVGR 49
Phosphoenolpyruvate carboxylase Eucgr.A01915.1 139.72 446 RLDIR 110
Heat shock protein 80 kDa Eucgr.J00025.1 13411, 211.262 131 NRTTPSYVAFTDTER 558 FELSGIPPAPR 80
Sucrose Synthase Eucgr.H03515.1 5361, 133.92 772 HVSNLDR 92
35S::PRMT10 Endochitinase A2 Eucgr.I01495.1 2131, 127.562 107 SFPAFGTTGDAATR 33
Beta Tubulin 5 Eucgr.D01847.1 1481 155 IREEYPDR 50
Elongation factor 1-alpha Eucgr.B02473.1 4981 165 ARYDEIVK 49
Heat shock protein 70 kDa Eucgr.J00023.1 3821, 204.212 38 NRTTPSYVAFTDTER 478 DNNLLGKFELSGIPPAPR, 540 TTPSYVGFTDTER 69

List of proteins identified from bands of whole cell lysates that correspond to methylated banding as determined by Western blotting. Score determined by either Mascot (1) or Peaks (−10logP) (2). Methylated arginines within sequences indicated in bold (dimethylated) or underlined (monomethylated)