Table 2.
Protein | Gene No. | Score | Methylated Peptide Sequences Identified by Mass Spectrometry | Predicted MW (kDa) | |
---|---|---|---|---|---|
35S::PRMT1 | Endoplasmin/Heat Shock protein 90 kDa | Eucgr.H00972.1 | 6111, 162.292 | 531 LGIIEDASNR | 93 |
RuBisCO large subunit- binding protein alpha | Eucgr.B02532.1 | 2181, 89.942 | 424 VGAATESELEDR | 69 | |
Alpha tubulin | Eucgr.B03604.1 | 851, 92.432 | 65 AIFLDLEPTVVDEVR | 49 | |
Glyceraldehyde-3-phosphate dehydrogenase | Eucgr.H04673.1 | 4991, 164.682 | 66 SISISISISIRPVR 346 RLEKAATYDEIK | 36 | |
Probable aldo-keto reductase | Eucgr.H04367.1 | 79.322 | 249 NRVVYAR | 38 | |
Armadillo/beta catenin repeat protein | Eucgr.B00340.1 | 21.522 | 377 IKLVKLNAVSALLGMLKSR | 58 | |
40S Ribosomal Protein | Eucgr.A02371.1 | 1171 | 194 VLQFAGIDDVFTSSR | 29 | |
Transaldolase family protein | Eucgr.G01978.1 | 2771 | 78 RTTLHDLYER | 47 | |
Probable Aldo-keto reductase | Eucgr.H04365.1 | 1062 | 106 GTPEYAR | 38 | |
ATP synthase subunit beta | Eucgr.G02224.1 | 1221 | 335 DAEGQDVLLFIDNIFR | 60 | |
Beta tubulin 5 | Eucgr.D01847.1 | 4811 | 155 IREEYPDR | 50 | |
Elongation factor 1-alpha | Eucgr.B02473.1 | 1791 | 244 IGGIGTVPVGR | 49 | |
Phosphoenolpyruvate carboxylase | Eucgr.A01915.1 | 139.72 | 446 RLDIR | 110 | |
Heat shock protein 80 kDa | Eucgr.J00025.1 | 13411, 211.262 | 131 NRTTPSYVAFTDTER 558 FELSGIPPAPR | 80 | |
Sucrose Synthase | Eucgr.H03515.1 | 5361, 133.92 | 772 HVSNLDR | 92 | |
35S::PRMT10 | Endochitinase A2 | Eucgr.I01495.1 | 2131, 127.562 | 107 SFPAFGTTGDAATR | 33 |
Beta Tubulin 5 | Eucgr.D01847.1 | 1481 | 155 IREEYPDR | 50 | |
Elongation factor 1-alpha | Eucgr.B02473.1 | 4981 | 165 ARYDEIVK | 49 | |
Heat shock protein 70 kDa | Eucgr.J00023.1 | 3821, 204.212 | 38 NRTTPSYVAFTDTER 478 DNNLLGKFELSGIPPAPR, 540 TTPSYVGFTDTER | 69 |
List of proteins identified from bands of whole cell lysates that correspond to methylated banding as determined by Western blotting. Score determined by either Mascot (1) or Peaks (−10logP) (2). Methylated arginines within sequences indicated in bold (dimethylated) or underlined (monomethylated)