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Primers used for RT-PCR to detect CTLA-4, Nanog mRNAs
| β-actin sense | GAA ATC GTG CGT GAC ATC AAA G |
| Antisense | TGT AGT TTC ATG GAT GCC ACA G |
| CTLA-4 sense | TCTGCAAGGTGGAACTCA |
| Antisense | GCTAACTG CGACAAGGAT |
| Nanog sense | ATCCCGAGAACTATTCTTG |
| Antisense | GGTACTTCTGCTTCTGAAAC |
