Fig. 5.

Restoration of PPM1A abundance partially reverses NS3-mediated enhancement of cell invasion. a–b After transfection with vector, NS3, NS3 and PPM1A, NS3 and PPM1A D239N, respectively, the migration and invasion capacities of Huh-7 were analyzed by wound closure (a) and transwell invasion (b) assays. c The protein levels of PPM1A, NS3, vimentin, E-cadherin and N-cadherin were measured by western blotting after transfection with plasmids indicated above. Data are the mean ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001 as evaluated using Student’s t-test