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. 2017 Mar 10;12:25. doi: 10.1186/s13024-017-0165-0

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 10 — 6 DIV primary cortical neurons derived from Tspan6 wt (Tspan6 ^+/Y) E14.5 embryos were infected with an empty lentiviral vector (LV-cnt) or a lentiviral vector overexpressing Tspan6 (LV-Tspan6). At 9 DIV neurons were washed and processed for EM analysis. Tspan6 wt neurons that overexpressed Tspan6 (e-h) show alterations by EM consisting of accumulation of electron dense material inside large vesicles (arrow) in the soma compared to control neurons (a-d)