Table 1. A summary of quantitative estimation of secondary structural elements of the GR AF1 and TBP before and after complex formation.
Spectrum | Helix, % | Sheet, % | Bend, % | Turn, % | Coil, % |
---|---|---|---|---|---|
AF1 | 26.7 | 11.6 | 10.0 | 12.3 | 39.4 |
AF1:TBP-TBP | 42.1 | 11.8 | 9.3 | 12.4 | 24.4 |
TBP | 41.3 | 16.1 | 9.4 | 12.4 | 20.8 |
AF1:TBP-AF1 | 41.0 | 16.4 | 9.6 | 12.4 | 20.6 |
The data are calculated from second-derivative FTIR spectra. AF1:TBP-TBP indicates the secondary structures attributable to AF1 in the AF1:TBP complex, after removal of the contributions of TBP; and AF1:TBP-AF1 indicates the structures of TBP in the complex after removal of the contribution of AF1.