Figure 4. Transmission electron micrographs of a rod-containing neurite showing the ~9 nm filament components in a cultured hippocampal slice treated 24 hr with Aβ_1–42 oligomers.

Slices were fixed, sectioned (250 nm) and stained as described elsewhere [Davis et al., 2009]. A rod runs the entire length of the neurite in the image shown (from lower left to upper right). Filaments of the rod surround a mitochondrion. White arrow points to 12 nm gold particle (slightly wider than the filament on which it sits), which was used as a fiduciary marker in reconstructing a tilted image stack to give the tomogram in panel (b). Scale bar = 100 nm.