Fig. 1.

Bar representation of the organization (Upper) and partial sequence alignments (Lower) of dinoflagellate LCFs. (Upper Top) Two copies of LCF genes arranged in tandem, separated by an intergenic region. (Upper Middle) The structure of Lp LCF, showing the N-terminal region followed by three repeated domains, D1, D2, and D3. (Upper Bottom) A diagram of the second domain of Lp subdivided into three regions: the N-terminal, the central, and the C-terminal. The central region (126–275) is more conserved than the flanking ones and is likely the catalytic core. (Lower) Alignment of sequences 30–70 of the N-terminal second domains of seven LCFs showing the four conserved histidines at positions 35, 45, 60, and 66.