Table 2. Predicted free energy levels for the RNA secondary structures folded from either the central region of the domains or the full domain.

Location	Aa	At	Pr	Lp	Pf	Pl	Pn
Central region
D1	-170.2 ± 0.6	-168.9 ± 0.6	-180.9 ± 0.4	-180.8 ± 0.4	-156.6 ± 0.5	-168.6 ± 0.2	-164.2 ± 0.3
D2	-177.4 ± 0.6	-171.0 ± 1.4	-184.1 ± 0.7	-172.8 ± 0.8	-168.3 ± 0.2	-164.9 ± 0.5	-167.5 ± 0.6
D3	-166.2 ± 0.8	-175.6 ± 1.5	-184.1 ± 0.7	-182.6 ± 0.7	-186.2 ± 0.6	-163.7 ± 0.5	-177.0 ± 0.3
Avg	-171.1 ± 5.7	-171.8 ± 3.4	-181.4 ± 2.4	-178.8 ± 5.3	-170.4 ± 14.9	-165.7 ± 2.6	-169.6 ± 6.7
Full domain
D1	-450.2 ± 2.2	-421.5 ± 0.5	-460.4 ± 0.6	-478.1 ± 0.5	-417.2 ± 1.7	-436.9 ± 1.4	-447.0 ± 0.3
D2	-432.2 ± 0.9	-431.4 ± 0.4	-491.6 ± 0.6	-460.3 ± 0.5	-424.8 ± 0.4	-406.3 ± 0.9	-422.0 ± 0.5
D3	-429.7 ± 1.7	-435.4 ± 0.5	-463.3 ± 0.3	-450.2 ± 0.9	-444.9 ± 0.7	-407.7 ± 0.5	-448.9 ± 1.7
Avg	-437.4 ± 11.2	-429.4 ± 7.2	-471.8 ± 17.2	-462.9 ± 14.1	429.0 ± 14.3	-417.0 ± 17.3	-439.3 ± 15.0

The mRNA sequences of the central regions (450 bases) or the full sequences of the individual domains from seven LCFs were imported into mfold (Version 4.0) for predicting the secondary structures (http://bioweb.pasteur.fr/seqanal/interfaces/mfold-simple.html). The five structures with the lowest free energy were used to obtain the average values for each sequence. Avg, averaged value of the three domains. The window sizes for folding the central regions and the full domains are 7 and 15, respectively. The predicted structures were viewed and printed with xrna (Version 1.1.10; http://rna.ucsc.edu/rnacenter/xrna/xrna.html).