Table 1.
Assay schedule and associated blood volumes for laboratory diagnostic tests performed during the study. A) Assays performed in all participants, and B), assays performed in participants reaching clinical or microbiological TD endpoint. Challenge: oral ingestion of 103 or 104 CFU S. Typhi Quailes strain suspended in 30 mL/0.53 g NaHCO3(aq). Antimicrobials: first-line, ciprofloxacin 500 mg twice daily for 14 days.
| A) All participants | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Day | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 28 |
| Hour | 0 | 6 | 12 | 0 | 12 | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Procedure | Challenge | Antimicrobials started | Antimicrobials completed | ||||||||||||||||
| Blood culture | – | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | – |
| Culture-PCR | – | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | |
| B) Additional time points in typhoid-diagnosed participants | ||||||||
|---|---|---|---|---|---|---|---|---|
| Timepoint + hours | TD + 0 | TD + 6 | TD + 12 | TD + 24 | TD + 36 | TD + 48 | TD + 72 | TD + 96 |
| Procedure | Antimicrobials started | |||||||
| Blood culture | 5 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
| Culture-PCR | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |