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. 2017 Feb 13;61(1):1273479. doi: 10.1080/16546628.2016.1273479

Figure 2.

Figure 2.

High-performance liquid chromatography (HPLC) profiles of major component of Solidago virgaurea var. gigantea (SV) ethanolic aqueous extracts and inhibitory effect of SV ethanolic aqueous extracts on 3T3-L1 adipocyte differentiation. (a) Chromatograms of SV ethanolic aqueous extracts; (b) contents of major components of SV ethanolic aqueous extracts; (c) effect of SV ethanolic aqueous extracts on viabilities of 3T3-L1 cells as determined by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay at 10 μg/ml for 24 h; (d) relative lipid content quantified by absorbance of 3T3-L1 cells treated with or without SV ethanolic aqueous extract at 10 μg/ml for 8 days; (e) microscope images of lipid droplets stained with Oil Red O in 3T3-L1 cells treated with or without SV ethanolic aqueous extract at 10 μg/ml for 8 days. Results are presented as mean ± SE. Asterisks indicate significant differences from differentiation medium (DM) (*p < 0.05, **p < 0.01, ***p < 0.001); daggers indicate significant differences from control (Con) (†p < 0.05, ††p < 0.01, †††p < 0.001). 1, protocatechuic acid; 2, chlorogenic acid; 3, 3,5-di-O-caffeoylquinic acid; 4, rutin; 5, unknown cinnamic acid derivative compound; 6, 1,3,5-tri-O-caffeoylquinic acid; 7, kaempferol-3-O-rutinoside; RT, retention time; SV0E, SV water extract; SV10E, SV 10% ethanol extract; SV30E, SV 30% ethanol extract; SV50E, SV 50% ethanol extract; SV70E, SV 70% ethanol extract; SV100E, SV 100% ethanol extract.