Skip to main content
. 2016 Nov 25;21(4):802–815. doi: 10.1111/jcmm.13022

Figure 5.

Figure 5

Reciprocal induction between p53 and SASH1 is induced in normal cells. (A) and (B) Exogenous p53 (HA‐p53) triggers exogenous SASH1 expression in a dose‐dependent manner. HEK‐293T cells and NHEMs were transfected with different amounts of HA‐p53 plasmid as indicated. Exogenous SASH1 RNA levels were measured by quantitative RT‐PCR and normalized to GAPDH. Expression of exogenous p53 protein and SASH1 was analysed by Western blot along with GAPDH as a loading control. (C) and (D) Exogenous SASH1 promotes expression of exogenous p53. Different amounts of GFP‐SASH1 plasmid and a certain amount of exogenous p53 were transfected to HEK‐293T cells and NHEMs cells and plasmid. Increasing amounts of GFP‐SASH1 trigger expression of exogenous p53, as analysed by QRT‐PCR and Western blot. (E) and (F) Exogenous SASH1 promotes expression of endogenous p53. Different amounts of exogenous SASH1 were introduced to HEK‐293T cells and NHEMs. After 36‐hr transfection, cells were lysed and subjected to Western blot to analyse the expression of GFP‐SASH1 as GAPDH as loading control. Results are the representative of three independent results. (G) An autoregulatory p53/POMC/Gαs/SASH1 loop mediates reciprocal induction of p53 and SASH1. p53 after being activated by different types of stress triggers the expression of POMC, Gαs and SASH1 successively. The induced SASH1 by p53/POMC/Gαs cascade promotes the up‐regulation p53 in nucleus, then induced nucleic p53 conversely activates POMC/Gαs/SASH1 cascade, which consists an autoregulatory p53/POMC/Gαs/SASH1 loop.