Figure 4.

A previously generated literature‐based list of manually selected human and murine genes, specifically expressed by either M1 or M2 macrophages, was used as a basis to test whether CDV infection of DH82 cells induces a polarization of canine macrophages in one of these categories. Gene expression data for the M1 and M2 category were compared between non‐infected and persistently CDV‐Ond‐infected DH82 cells, employing multiple pairwise nonparametric Mann–Whitney U‐tests. Differential expression was defined by the combination of a statistical significance filter (Mann–Whitney U‐test; P ≤ 0.05) and a fold change filter (FC ≥ 2.0 or ≤ −2.0). A total of 21 of the 59 unique canine gene symbols for the M1 category were differentially regulated (A; red and marked by asterisks), whereas 19 of 55 genes exhibited differential regulation for the M2 category (B; green and marked by asterisks), respectively.