Figure 1.

Work flow of sample collection and processing for HPV analysis and expression analysis for transcription factors. Prospectively collected, paired fresh and formalin‐fixed tissues, and retrospective tissue sections were evaluated for optimization of protocol for detection of HPV infection and expression of transcription factors in oral cancer cases by molecular methods and IHC. *Four fresh tissues and 10 paraffin blocks of noncancerous patients were also collected as controls with prior consent from dental clinic and processed similarly for HPV detection and IHC not depicted in the diagram.