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. 2017 Jan 24;230(4):567–574. doi: 10.1111/joa.12583

Figure 2.

Figure 2

Intestinal smooth muscle differentiation is independent of enteric nervous system (ENS) formation. Ganglionic (A,C) and aganglionic (B,D) E5 chick hindgut was grafted onto the choriallantoic membranes (CAM) and assessed for smooth muscle actin (SMA) (A,B) and calponin (C,D) immunoreactivity 9 days later. N‐Cadherin (Ncad) (A,B) and Tuj1 (C,D) were used to detect enteric neural crest‐derived cells (ENCCs) and differentiated neurons, respectively. Smooth muscle development in the aganglionic hindgut progressed normally in the absence of the ENS (B,D) and was comparable to that of normally ganglionated intestine (A,C). Scale bar in (A) corresponds to 120 μm (A,B) and 60 μm (C,D). Calp, calponin; cm, circular muscle; mm, muscularis mucosae; Ncad, N‐cadherin.