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Smooth muscle inhibitors do not affect enteric neural crest‐derived cell (ENCC) proliferation or survival. E5 guts were cultured for 3 days in the presence of FK‐506 and AG‐1295 (A,C) or dimethylsulfoxide (DMSO) as control (B,D). ENCC proliferation was detected by EdU incorporation in Ncad+ cells (A,B). Cell apoptosis was detected as immunoreactivity to activated caspase‐3 (C,D). Scale bar in (A) corresponds to 60 μm.
