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. 2016 Dec 20;21(4):768–780. doi: 10.1111/jcmm.13019

Figure 1.

Figure 1

Influence of retinoschisin deficiency on MAP kinase signalling in the murine retina. C‐Raf and Erk1/2 phosphorylation in murine wild‐type and Rs1h −/Y retinae, harvested at post‐natal days 7, 10 and 14 (P7, P10 and P14). Retinal lysates were subjected to Western blot analyses with antibodies against phosphorylated c‐Raf (pRaf), total c‐Raf (Raf), phosphorylated Erk1 and Erk2 (pErk1/2), total Erk1 and Erk2 (Erk1/2), as well as ActB as a control (A). Densitometric quantification (B) was performed with immunoblots from three independent sample sets. Signals for pErk1/2, Erk1/2, pRaf and Raf were normalized against ActB and calibrated against signals for wild‐type retinae. Data represent the mean + S.D. (C) C‐Fos and Egr1 expression in murine wild‐type and Rs1h −/Y retinae harvested at post‐natal days 7, 10 and 14 (P7, P10 and P14). mRNA expression of C‐Fos and Egr1 was determined via quantitative real‐time RT‐PCR. Five independent sample sets were analysed. Results were normalized to Hprt transcript levels and calibrated with the control. The mean + S.D. for the three (immunoblot analyses) or five (quantitative RT‐PCR) independent sample sets is given. Asterisks mark statistically significant (*P < 0.05) and highly significant (**P < 0.01) differences.