Figure 4.

H3Lys4 methylation characterization at the AQY1 locus (A). ChIP-qPCR was performed on wt, jhd2Δ, cnc1Δ, and cnc1Δjhd2Δ mutants cultured in either fermentable (B) or nonfermentable (C) carbon sources. Immunoprecipitations were carried out using antibodies that recognize histone H3, or each individual H3Lys4 methylation level (1me, 2me, or 3me). To adequately map H3Lys4 methylation abundance across the AQY1 locus, qPCR reactions were performed using four separate amplicons (I–IV). Histograms represent the average of two independent biological replicates, error bars indicate SD. ChIP, chromatin immunoprecipitation; H3Lys4, histone H3Lys4; 1me, monomethylation; 2me, dimethylation; 3me, trimethylation; qPCR, quantitative polymerase chain reaction; wt, wild type.