Figure 5.

CDK8-dependent Set1p occupancy at the AQY1 locus. ChIP-qPCR was performed on wild type or cdk8Δ mutant yeast harboring a chromosomally integrated 9-myc tag at the SET1 locus. Yeast were cultured to midlogarithmic phase in either (A) fermentable or (B) nonfermentable carbon sources. Immunoprecipitations were performed using myc-conjugated agarose beads with wild-type untagged yeast serving as a negative control. qPCR reactions were directed at the amplicons I–IV that span the AQY1 locus as described in Figure 4. Ab, antibody; ChIP, chromatin immunoprecipitation; IP, immunoprecipitation; qPCR, quantitative polymerase chain reaction.