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. 2017 Jan 30;7(3):1049–1060. doi: 10.1534/g3.117.039065

Figure 1.

Figure 1

Activated Yki in endoreplicating cells shows distinct nuclear sizes in the pouch and hinge regions. (A) Diagram of Drosophila larva, showing the imaginal wing disc in its third-instar larval stage. (B) Larval development timeline (hours AED). Heat shock was applied at 48 hr AED and dissection was administered 120 hr AED. (C–D’’) Overexpression of Fzr and ykiM123 (C–C’’): the difference in the nuclear size in the pouch and hinge region. Overexpression of Fzr (D–D’’) as a control. Yellow dotted lines outline the pouch. The scale bar is 20 cm. (E and E’) Quantifications of the nuclear size, demonstrating that Fzr/ykiM123 overexpression results a larger nuclear size in the pouch than the hinge, whereas Fzr overexpression does not show significant difference [n(Fzr/ykiM123) = 31, n(Fzr) = 62 (pouch), and 26 (hinge); error bars are SE values]. AED, after egg deposition; Fzr, Fizzy-related; GFP, green fluorescent protein; Yki, Yorkie.