Figure 5.

Yki regulates CycE during development through temporally expressed Sd. (A) Timeline of Sd expression during larval development, noting that Sd is predominantly expressed in the wing pouch region. (B) A wing disc growth curve depicts the relationship between the overall size of the wing (in log) and time (hours AED) of development. A regression equation (shown in a dotted line), y = 0.0547x + 8.2974, is built with an R-square = 0.89. Error bars are SE values. (C–F’) Expression patterns of Sd and Hth in the wing disc of the late and early third-instar larval stage (L3). Sd is expressed predominantly in the wing pouch in late L3 (C–C’) and begins to emerge in early L3 [(E and E’), arrows show the emergence of Sd]. Hth is primarily expressed in the hinge in late L3 (D and D’) and ubiquitously expressed in early L3 (F and F’). The transverse view shows the structure of the wing disc, helping determine the localization of Sd and Hth. The scale bar is 50 cm. (G) Timeline of Sd expression in the wing pouch is established by the wing disc growth curve and Sd staining. Error bars are SE values. (H–I’’) The temporal phenotypes of Fzr/yki^M123 and Fzr/yki^M123/Sd cells were examined in the early stage of larval development. The scale bar is 20 cm. (J) Quantifications of NEIs of Fzr/Yki^M123 overexpression in the pouch and hinge in the early stage (n = 72, error bars are SE values). (K) Quantifications of NEIs of Fzr/yki^M123 and Fzr/yki^M123/Sd overexpression in the early stage [n(Fzr/yki^M123) = 72 and n(Fzr/yki^M123/Sd) = 20; error bars are SE values]. AED, after egg deposition; CycE, Cyclin E; DAPI, 4’,6-diamidino-2-phenylindole; Fzr, Fizzy-related; GFP, green fluorescent protein; Hth, Homeothorax; NEI, nuclear enlargement index; Sd, Scalloped; Yki, Yorkie.