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. 2017 Jan 17;7(3):789–799. doi: 10.1534/g3.116.037366

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Copyright © 2017 Duncan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Autophagy is blocked in Idh3b-mutant salivary gland cells. All images show salivary gland cells from 8 to 12 APF prepupae, in which expression of UAS-Atg8a-GFP is driven by Sg-Gal4. Atg8a-GFP is shown in green, and LysoTracker staining is in red. (A–C) In Idh3b⁺/Df(3R)Exel6188 cells, Atg8a-GFP labeled vesicles are densely packed, and these vesicles colabel with LysoTracker, identifying them as autolysosomes. (D–I) In Idh3b¹/Df(3R)Exel6188 and Idh3b²/Df(3R)Exel6188 cells, Atg8a-GFP-labeled vesicles are dramatically reduced in number, and LysoTracker staining is much reduced, indicating an almost complete block in autophagy. (J–L) Idh3b³/Df(3R)Exel6188 cells show an intermediate phenotype, in which Atg8a-GFP vesicles are partially formed, and LysoTracker staining is weakened, and incompletely associated with Atg8a-GFP fluorescence.