Figure 2.

SDL interactions involving loss-of-function kinase mutations and overexpression alleles of TFs. Network diagram showing 68 confirmed GIs between 52 TF overexpression alleles and four loss-of-function kinase alleles (pho85-as, cdc28-as, slt2Δ, cka2Δ; orange nodes). TF overexpression alleles are represented as nodes connected to kinase alleles by edges, which indicate either positive (blue line; arrowhead indicating direction of suppression) or negative (black line) GIs. Evidence in the literature showing TF phosphorylation by the interacting kinase is indicated by node color: yellow (in vitro); light green (in vivo); and dark green (both in vivo and in vitro). GIs, genetic interactions; SDL, synthetic dosagelethality; TF, transcription factor.