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. 2017 Jan 24;7(3):991–1000. doi: 10.1534/g3.116.037515

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Copyright © 2017 Gee et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Sizes of endolysosomal compartments. (A) Confocal images of coelomocytes in young adult hermaphrodites of the indicated genotypes that also carry the cdIs131[Pcc1::GFP::rab-5; unc-119-myo-2::GFP] transgene. The scatter plot shows the sizes of individual GFP::RAB-5-labeled compartments in the coelomocytes. (B) Confocal images of coelomocytes in young adult hermaphrodites of the indicated genotypes that also carry the cdIs153[rme-8::GFP; unc-119-myo-2::GFP] transgene. The scatter plot shows the sizes of individual RME-8::GFP-labeled compartments in the coelomocytes. (C) Confocal images of coelomocytes in young adult hermaphrodites of the indicated genotypes that also carry the cdIs40[Pcc1::GFP::cup-5; unc-119-myo-2::GFP] transgene; the cup-5 mutant carries the pwIs50[lmp-1::GFP; unc-119] transgene. The left scatter plot shows the sizes of individual GFP::CUP-5-labeled compartments in the coelomocytes; the cup-5 mutant is shown on a separate chart to adjust for scale. The black circle indicates the average size of organelles. The number above each column indicates the number of organelles measured for each strain. Inverted brackets indicate P < 0.05 of mutants compared to wild type. Bar = 5 µm.