Fig 5.

Secretion of HMGB1 in LLC (A) and B16F10 (B) cell lines. LLC and B16F10 cells were treated with R2016 (0.5, 1.5, 2.5 μg/ml) or doxorubicin (0.01 μg/ml) for 18 hr and cultured supernatants were harvested. HMGB1 protein concentrations in cultured supernatants from LLC and B16F10 cells were measured by ELISA. Data are presented as mean±SE. Significant effects compared to untreated-control (*p<0.05, **p<0.01, ***p<0.005) and doxorubicin treated positive control (#p<0.05, ##p<0.01, ###p<0.005).