Fig 4. The switch from the A2 to the A3 pathway is not dependent on RNA pol I.

(A) Northern blot analysis of the pre-rRNA processing in the strain NOY892, grown in YP-galactose. Hybridization probes as described in Fig 1A and 1B. (B) NOY892 was treated with 200 ng/ml rapamycin at OD₆₀₀ = 2 or exposed to heat shock by shifting from 25°C to 37°C. (C) Northern blot analysis of pre-rRNA processing in the anchor-away strain BEN135, expressing Rpa135–FRB fusion treated with rapamycin at OD₆₀₀ = 2. (D) Northern blot analysis of the pre-rRNA processing in the CARA strain treated with rapamycin OD₆₀₀ = 2.