Table 3. Comparison of taxonomic distribution of reads between V1-V3 and V3-V4 target regions.
| Phylum | V1-V3 region | V3-V4 region | ||||
|---|---|---|---|---|---|---|
| OTU count | Read count | Percent | OUT count | Read count | Percent | |
| Acidobacteria | 5 | 167 | 1.01% | 20 | 640 | 1.83% |
| Actinobacteria | 15 | 844 | 5.10% | 31 | 2303 | 6.57% |
| Armatimonadetes | 2 | 10 | 0.06% | 5 | 113 | 0.32% |
| Bacteroidetes | 23 | 259 | 1.57% | 124 | 5732 | 16.36% |
| Candidate division WPS-1 | 0 | 0 | 0% | 1 | 13 | 0.04% |
| Candidatus Saccharibacteria | 0 | 0 | 0% | 1 | 20 | 0.06% |
| Chlamydiae | 0 | 0 | 0% | 1 | 2 | 0.01% |
| Chloroflexi | 0 | 0 | 0% | 3 | 6 | 0.02% |
| Cyanobacteria | 6 | 6958 | 42.05% | 10 | 10834 | 30.93% |
| Firmicutes | 1 | 65 | 0.39% | 1 | 378 | 1.08% |
| Gemmatimonadetes | 0 | 0 | 0% | 3 | 96 | 0.27% |
| Hydrogenedentes | 0 | 0 | 0% | 1 | 37 | 0.11% |
| Nitrospirae | 0 | 0 | 0% | 1 | 12 | 0.03% |
| Parcubacteria | 2 | 10 | 0.06% | 6 | 28 | 0.08% |
| Planctomycetes | 8 | 144 | 0.87% | 24 | 1230 | 3.51% |
| Proteobacteria* | 121 | 6201 | 37.47% | 185 | 9534 | 27.22% |
| Spirochaetes | 1 | 9 | 0.05% | 0 | 0 | 0% |
| Unclassified Bacteria | 69 | 1852 | 11.19% | 47 | 1039 | 2.97% |
| Verrucomicrobia | 2 | 29 | 0.18% | 38 | 3011 | 8.60% |
| Total | 255 | 16548 | 100% | 502 | 35028 | 100% |
* Please refer to Table 4 for class level breakup of phylum Proteobacteria.