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. 2017 Mar 10;12(3):e0173264. doi: 10.1371/journal.pone.0173264

Fig 5. Effects of coffee extract on MDI-induced phosphorylation of IRβ, MEK, ERK, and Akt in 3T3-L1 cells.

Fig 5

3T3-L1 cells were treated with 5% (v/v) coffee extract for 1 hr, and then stimulated with MDI for the indicated periods. (A) Whole cell lysates were immunoblotted with an anti-phospho-IRβ antibody or anti-IRβ antibody. The relative phosphorylation of IRβ is shown in the graph. Values are the mean ± S.D. of three independent experiments. *p<0.01, ***p<0.001 vs. untreated cells. (B) Whole cell lysates were immunoblotted with an anti-phospho-MEK antibody, anti-MEK antibody, anti-phospho-ERK1/2 antibody, anti-ERK antibody, anti-phospho-Akt antibody, or anti-Akt antibody. (C) The phosphorylation levels of MEK, ERK1, ERK2, and Akt were normalized with their protein amounts. The relative phosphorylation of MEK, ERK1, ERK2, and Akt is shown in the graphs. Values are the mean ± S.D. of three independent experiments.