Skip to main content
. 2017 Mar 10;12(3):e0173264. doi: 10.1371/journal.pone.0173264

Fig 7. Effects of coffee extract on the stability of IRS1 in 3T3-L1 cells.

Fig 7

(A) 3T3-L1 cells were treated with cycloheximide (CHX) (100 μg/mL) in the presence and absence of coffee extract (5% (v/v)) for the indicated periods. Cell lysates were immunoblotted with an anti-IRS1 antibody or anti-β-actin antibody. The expression level of IRS1 and β-actin was quantified and shown in the graphs. Values are the mean ± S.D. of three independent experiments. (B) 3T3-L1 cells were pretreated with DMSO (0.1%) or MG132 (20 μM) for 1 hr prior to the treatment with coffee extract (5% (v/v)) for 1 hr. Cell lysates were immunoblotted with an anti-IRS1 antibody or anti-β-actin antibody. The expression level of IRS1 was normalized to the protein amount of β-actin. The relative expression level of IRS1 is shown in the graphs. Values are the mean ± S.D. of three independent experiments. *p<0.05; ***p<0.001 significantly different from the control group treated with DMSO. ##p < 0.01 significantly different from the group treated with DMSO and coffee.