Table 1. Primers for gene expression analysis used in this study.
| Gene | Sequence (5'->3') | Amplicon (bp) | Tm | NCBI Gene ID | |
|---|---|---|---|---|---|
| MITF | F | CCCAGTTCATGCAACAGAGAG | 79 | 58.9 | 4286 |
| R | GCAGAGGGAAGGGTGGTG | 59.7 | |||
| RPE65 | F | CGTATGGACTTGGCTTGAATC | 129 | 57.1 | 6121 |
| R | CTGGGTGAGAAACAAAGATGG | 56.8 | |||
| PMEL17 | F | TGATGGCTGTGGTCCTTGC | 94 | 60.3 | 6490 |
| R | CAGTGACTGCTGCTATGTGG | 58.6 | |||
| PEDF | F | TATCACCTTAACCAGCCTTTCA | 83 | 57.1 | 5176 |
| R | GGGTCCAGAATCTTGCCAATG | 59.3 | |||
| TYRP1 | F | GATTCCACTCTAATAAGCCCAAA | 198 | 56.3 | 7306 |
| R | TTCCAAGCACTGAGCGACAT | 60.0 | |||
| BEST1 | F | TAGAACCATCAGCGCCGTC | 261 | 60.0 | 7439 |
| R | TGAGTGTAGTGTGTATGTTGG | 55.3 | |||
| RBP1 | F | CAGGTTGGGAAGGAGTTTGA | 168 | 57.3 | 5947 |
| R | TGGTGGCGTGTGACTGTAAT | 59.6 | |||
| PAX6 | F | TGTGTGCTCTGAAGGTCAGG | 170 | 59.6 | 5080 |
| R | CTGGAGCTCTGTTTGGAAGG | 58.2 | |||
| GAPDH | F | GAGTCAACGGATTTGGTCGT | 185 | 58.2 | 2597 |
| R | GACAAGCTTCCCGTTCTCAG | 58.6 | |||
| GAPDH | F | GTGGACCTGACCTGCCGTCT | 153 | 61.7 | 2597 |
| R | GGAGGAGTGGGTGTCGCTGT | 61.7 | |||
| OCT4 | F | CCTCACTTCACTGCACTGTA | 164 | 54.3 | 5460 |
| R | CAGGTTTTCTTTCCCTAGCT | 52.2 | |||
| NANOG | F | CAGAAGGCCTCCGCACCTAC | 111 | 60.2 | 79923 |
| R | ATTGTTCCAGGTCTGGTTGC | 55.6 | |||
| SOX2 | F | GGGAAATGGGAGGGGTGCAAAAGAGG | 151 | 63.6 | 6657 |
| R | TTGCGTGAGTGTGGATGGGATTGGTG | 63.1 | |||
| LIN28 | F | ACCGGACCTGGTGGAGTATT | 133 | 58.0 | 79727 |
| R | CTGGGGATGCCTTAGCAAC | 58.4 | |||
| HDNMT3B | F | CAAATGAACCCTGCGCTGTC | 207 | 57.1 | 1783 |
| R | CAGGTCCCCAGATTCCCTCA | 58.6 | |||
| REXO1 | F | CCCACGTGCGCTGATCTCT | 134 | 60.1 | 57455 |
| R | CTGAGGTAGTCGGCCATGAG | 57.1 | |||
| TERT | F | CTCAGGACAGGCAAGTGTGG | 232 | 58.1 | 7015 |
| R | ATGGGAAGCAGGGGTTCAAG | 57.6 |